NEUREST Training Areas

Training Area	Methods and Skills	Faculty
Molecular Biology	Cloning, transfections, transformations, PCR-based techniques, oligonucleotide- and vector-based siRNA-knockdowns, DNA microarray systems	Brose, Jahn, Missler, Nave, Chowdhury, Sigrist
Genetics	Transgenic animals (Drosophila, mice) incl. conditional knock-out and knock-in strategies, mutagenesis and mutant screens, embryonic stem cells	Brose, Bähr, Krieglstein, Nave, Mansouri, Missler, Sigrist,
Protein Chemistry	Protein expression and purification, structural biology (crystallography and NMR), mass spectrometry and proteomics, fluorescence and CD-spectroscopy, calorimetry, immunochemistry (polyclonal, monoclonal, and recombinant antibodies)	Brose, Fasshauer, Griesinger, Jahn, Martens
Cell Biology	Organelle isolation and characterization, high-resolution microscopy imaging techniques including fluorescence and time-resolved and live cell techniques, calcium imaging, multiphoton confocal microscopy, total internal reflection fluorescence microscopy, electron microscopy	Klingauf, Jahn, Neher, Rupnik, Sigrist, Wouters, Schneggenburger, Stühmer, Schild, Schürmann
Cell and Organ Culture	Culturing of primary neurons and glial cells, organotypic cultures, brain slice cultures	Neher, Rupnik, Stoykowa, Neumann, Richter, Schild
Electrophysiology	Standard intra- and extracellular recording, patch-clamp recording, amperometry,	Neher, Schneggenburger, Rupnik, Stühmer, Terlau, Richter, Moser, Schild
Neuroanatomy	Structural and functional neuroanatomy, Immunochemical techniques, in-situ hybridization, laser-microdissection	Krieglstein, Stoykova, Schürmann, Fuchs, Flügge, Elsner
Clinical Neuroscience	Neuropathology, functional imaging, spatially resolved NMR, transcranial magnetic and direct stimulations, animal models for human neurological disorders, neuropharmacology, access to clinical diagnosis and treatment facilities	Bähr, Ehrenreich, Frahm, Paulus, Fuchs, Moser, Knepel, Gärtner
Theoretical and Computational Neuroscience	Modeling, Monte-Carlo simulations, statistics, bioinformatics	Geisel, Zippelius, Brunner, Schild